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Aliquots of a 0.5-mg/mL standard of BSA are used to construct a standard curve for the Bradford protein assay. The tubes contain the following amounts of the BSA solution: 0, 20, 40, 60, 80, and 100 µL. The corresponding absorbencies after adding Bradford reagent are 0, 0.05, 0.09, 0.14, 0.19, and 0.22. If you take 20 µL of an unknown and add 80 µL of water, mix, take 10 µL of the mixture and add to Bradford reagent, and see an absorbance of 0.08, what is the protein concentration of the undiluted unknown?

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