Acid phosphatase was assayed at s 2x10-5 m the km for the, Ask an Expert

Academics

Acid Phosphatase was assayed at [S] = 2x10-5 M. The Km for the substrate was 5x10-4 M. At the end of one minute 2.5% of the substrate, pNPP, had been converted to pNP (a product). Answer the following questions using this information:

a) What percent of pNPP will be converted at the end of 3.5 minutes assuming product formation is in 1st order of reaction?
b) What will be the product concentration at the end of 3.5 minutes?
c) Determine maximal velocity (Vmax) with the enzyme concentration used.

View complete question

Chemistry, Academics

Category:- Chemistry
Reference No.:- M91022859

Have any Question?Write your Review or question?

Related Questions in Chemistry

When benzene c6h6 reacts with bromine br2 bromobenzene

When benzene (C6H6 ) reacts with bromine (Br2 ), bromobenzene (C6H5Br ) is obtained: C6H6+Br2→C6H5Br+HBr What is the theoretical yield of bromobenzene in this reaction when 28.0 g of benzene reacts with 60.7 g of bromine ...

When 356 g of an unknown hydrocarbon was dissolved in 125 g

When 3.56 g of an unknown hydrocarbon was dissolved in 125 g of benzene, the freezing pointof the solution was found to be 4.41oC. Analysis of thehydrocarbon showed that it contained 93.71% carbon (by mass) and6.29% hydr ...

How many moles of magnesium ion are present in 525g

How many moles of magnesium ion are present in 5.25g Mg 3 (PO 4 ) 2 ?

Using data from unfiltered white light and the white light

Using data from unfiltered white light and the white light through the yellow filter, determine which two colors of light mix to form yellow. Support your conclusion with a quantitative discussion of relative peak intens ...

Why does a hydrate exist only as a solid what

Why does a hydrate exist only as a solid? What characteristic of a hydrate is responsible for this phenomenon?

A 315-nbspsample ofnbspnh4no3nbspis introduced intoan

A 3.15- sample of NH4NO3 is introduced intoan evacuated 2.26- L flask and then heated to 245 C. What is the total gas pressure, in atmospheres, in the flask at245 C when the NH4NO3 has completely decomposed?

Protein folding assignmentin an in vitro protein folding

PROTEIN FOLDING ASSIGNMENT In an "in vitro" protein folding experiment monitored by fluorescence measurements the following data was obtained for protein X: [GuHCl], M Wavelength Maxima, nm 0 330 0.5 332 1.0 331 1.5 332 ...

Insoluble pbbr2snbspnbspprecipitates when solutions of

Insoluble PbBr2(s) precipitates when solutions of Pb(NO3)2(aq) and NaBr(aq) are mixed. Pb(NO3)2(aq) + 2NaBr(aq) ---> PbBr2(s) + 2NaNO3(aq) ΔrH = ? To measure the enthalpy change, 350. mL of 0.50 M Pb(NO3)2(aq) and 350. ...

Anbsp00500-molnbspsample of a nutrient substance is burned

A 0.0500-mol sample of a nutrient substance is burned in a bomb calorimeter containing 2.00 × 10 2 g H 2 O. If the molar mass of this nutrient substance is 114 g/mol, what is the fuel value (in nutritional Cal) if the t ...

How many formula units ofnbspna2so4are present in

How many formula units of Na2SO4are present in a 450 gram sample of the salt? Use 6.022×1023mol-1 or Avogadro's number. Report your answer in scientific notation. Your answer should have two significant figures.

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Chemistry Expert