Acetyl-CoA carboxylase is a key regulatory enzyme in fatty acid synthesis. Early studies on the molecular properties of this enzyme purified from avian liver produced the data given in table.
Treatments
|
Sedimentation coefficient (S)
|
Molecular mass (kDa)
|
Catalytic activity (arbitrary units)
|
10 mM citrate
|
55-59
|
4000-8000
|
35
|
0.5M NaCl, pH8-9
|
13.1
|
410
|
2.5
|
l) Explain the results shown in table in terms of the molecular structure of acetyl-CoA carboxylase and comment on their apparent physiological significance.
l) Which cellular compartment would you expect the acetyl-CoA carboxylase activity to have been observed in and why?