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A sample of cells with a total receptor concentration of 50 mM was incubated with a ligand concentration of 100 mM. Following the incubation period, the concentration of unbound receptors was determined to be 15 mM. What is the Kd for the receptor-ligand interaction?

A) 28 mM

B) 43 mM

C) 143 mM

D) 217 mM

E) 333 mM

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