A reaction mixture containing 20 mm o-acetyl serine 50 mm, Ask an Expert

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A reaction mixture containing 20 mM O-acetyl serine, 50 mM NaCl, 50 mM HEPES buffer (pH 7.6), and O-acetyl serine acetylase is incubated in 37C until O-acetyl serine is completely hydrolyzed. What is the pH of the mixture at the end of the reaction?
O-acetly serine, pKa1=1.9, pKa2=8.6
serine, pKa1=2.2, pKa2=9.2
acetic acid pKa=4.76
HEPES, pKa1=3 pKa2=7.6

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