A mutant hemoglobin has His146 of the beta chain replaced with a threonine residue. predict what the consequence of this mutation might be. specifically:
(A) would you expect a change in p50? If so, which way?
(B) would you expect a change in cooperativity? If so, which way?
(C) Would you expect a change in DPG binding? If so, which way?
(D) Would you expect the hemoglobin by electrophoresis at pH5? At pH 7?