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A mixture containing 18.8uM of the analyte of interest and 44.4uM of the internal standard produced chromatographic peak areas of 306 for the analyte and 511 for the standard. A second solution containing an unknown quantity of the analyte as well as 55.5 uM of the internal standard produced peak areas of 251 for the analyte and 563 for the internal standard.

Explain what is the concentration of the analyte in the second solution expressed as uM?

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