Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Chemistry Expert

problem 1:

A solution needs 250 ml of a 7.5% (w/v) solution of tri sodium citrate. The only citrate you encompass available is the dehydrate, C6H5O7Na3. 2H20 through a formula weight of 294.1.How much would you encompass to make up this solution?

problem 2:

A citrate buffer should be made up to a final concentration of 50mM. You require making up 2L. You have to stock solutions from which to make this buffer; 0.1 M citric acid (C6H8O7) and 0.1 M Sodium citrate (C6H5O7Na3). You require adding twice as much of the acid form than the base form to accomplish the desired pH. Illustrate how will you make up this solution?

problem 3:

To isolate RNA you frequently use a protein denaturant solution of guanidinum salts. Such chaotropic solutions denature through disrupting H-bonding. The solution also contains a reducing agent to denature the disulfide bonds. The final composition of the solution is: 4 guanidine isthiocyanate (GITC), 25mM sodium citrate, pH 7.0, 0.5% (w/v) SDS, 100mM β-mercaptoethanol. You have guanidine isothiocyanate powder (mol.Wt.118.16),1 M NaCitrate, pH 7.0, powder SDS and b-mercaptoethanol (density 1.12 g/mL, mol. Wt.78.1). How would you build up 100 mL of this solution?

Chemistry, Academics

  • Category:- Chemistry
  • Reference No.:- M99174

Have any Question? 


Related Questions in Chemistry

A coffee cup calorimeter having a heat capacity of 451 joc

A coffee cup calorimeter having a heat capacity of 451 J/ o C was used to measure the heat evolved when 0.0300 mol of NaOH(s) was added to 1000 mL of 0.0300 M HNO 3 initially at 23.000 o C. The temperature of the water r ...

1 perchloric acid is either a broslashnsted-lowry acid or a

1. Perchloric acid is either a Brønsted-Lowry acid or a Brønsted-Lowry base. If it is an acid, what is it's conjugate base? On the other hand, if it is a base what is it's conjugate acid? 2. Phosphate ion is either a Brø ...

At 518degc the rate of decomposition of a sample of gaseous

At 518°C, the rate of decomposition of a sample of gaseous acetaldehyde, initially at a pressure of 363 Torr, was 1.07 Torr s-1 when 5.0% had reacted and 0.76 Torr s-1 when 20.0% had reacted. If the reaction rate is prop ...

The combustion of so2g has keq560x104 at 350 degrees

The combustion of SO2(g) has Keq=5.60x10^4 at 350 degrees Celcius. If 0.350bar of SO2(g) and 0.762bar of O2(g) are mixed initially, what are the partial pressures of each of the three gases when the mixture reaches equil ...

1a rolaids tablet has 550 mg of calcium carbonate and 110

1. A Rolaids tablet has 550 mg of calcium carbonate and 110 mg of magnesium hydroxide. a) Write the two neutralization reactions with stomach acid (assume HCl, don't combine reactions) b) If the acid concentration is 4.0 ...

You are tasked to determine the amount of blue dye in a

You are tasked to determine the amount of blue dye in a sample of purple energy drinka a) How would you be able to see only the blue dye to determine concentration when the drink itself is purple? b) Describe the procedu ...

A cleaning solution is found to have a oh- 32 x 10-12m

A cleaning solution is found to have a [OH-] = 3.2 x 10 -12 M. What is the [H 3 O + ] and is the solution acidic or basic?

The plate height in a particularly packed gas

The plate height in a particularly packed gas chromatography column is characterized by the van Deemter equation with parameters: A= 1.50 mm, B= 25.0 mm ml/min, and C = 0.0250 mm min/mL. a) Please use spreadsheet to cons ...

When 100 moles of hig is sealed in 100l flask at 225

When 1.00 moles of HI(g) is sealed in 1.00L flask at 225 degrees Celcius, it decomposes until the equilibrium amount of I2(g) is 0.182 moles. Calculate Keq using this information. The decomposition reaction is HI(g)=I2(g ...

Using the debye-hucket equation calculate the activity

Using the Debye-Hucket equation, calculate the activity coefficients for the acidic and basic forms of bromocresol green, HX- and X2-, for a solution with an ionic strength of u=0.1M.

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

WalMart Identification of theory and critical discussion

Drawing on the prescribed text and/or relevant academic literature, produce a paper which discusses the nature of group

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro