Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Chemistry Expert

problem 1:

A solution needs 250 ml of a 7.5% (w/v) solution of tri sodium citrate. The only citrate you encompass available is the dehydrate, C6H5O7Na3. 2H20 through a formula weight of 294.1.How much would you encompass to make up this solution?

problem 2:

A citrate buffer should be made up to a final concentration of 50mM. You require making up 2L. You have to stock solutions from which to make this buffer; 0.1 M citric acid (C6H8O7) and 0.1 M Sodium citrate (C6H5O7Na3). You require adding twice as much of the acid form than the base form to accomplish the desired pH. Illustrate how will you make up this solution?

problem 3:

To isolate RNA you frequently use a protein denaturant solution of guanidinum salts. Such chaotropic solutions denature through disrupting H-bonding. The solution also contains a reducing agent to denature the disulfide bonds. The final composition of the solution is: 4 guanidine isthiocyanate (GITC), 25mM sodium citrate, pH 7.0, 0.5% (w/v) SDS, 100mM β-mercaptoethanol. You have guanidine isothiocyanate powder (mol.Wt.118.16),1 M NaCitrate, pH 7.0, powder SDS and b-mercaptoethanol (density 1.12 g/mL, mol. Wt.78.1). How would you build up 100 mL of this solution?

Chemistry, Academics

  • Category:- Chemistry
  • Reference No.:- M99174

Have any Question? 


Related Questions in Chemistry

A soil analysis was done for the exchangeable cations in

A soil analysis was done for the exchangeable cations in the soil and the results were as follows:Ca = 1.8 g/kg (atomic wt. Calcium is 40 g/mol)Mg = 3.25 cmolK = 3 cmolNa = 0.230 g/kg (atomic wt. of Na is 23 g/mol)H = 9 ...

Write a reserch paper on gas chromatographythis is a 10

Write a reserch paper on Gas chromatography. This is a 10 pages research paper that should include an introduction on chromatography, principles of separation, type of columns, resolution, detection methods and applicati ...

Consider a pipe with a length of 435 cm if the temperature

Consider a pipe with a length of 43.5 cm. If the temperature of the air is 19.5 oC and the pipe is closed in one end and open in the other, what is the frequency of the third harmonic for the pipe? Post your answer in he ...

A solution containing the complex formed between biiii and

A solution containing the complex formed between Bi(III) and thiourea has a molar absorptivity of 9.32x10^3 Lxcm^-1xmol^-1 at 470 nm. What is the absorbance of a 6.24 x10^-5 M solution of the complex in a 1.00-cm cell? W ...

Suppose 68 g of carbon monoxide is reacted with 8g of

Suppose 68 g of carbon monoxide is reacted with 8g of hydrogen gas to produce methanol (chemical formula CH3OH or CH4O) a) Calculate the theoretical yield of methanol. b) If 3.57 x 10^4 of methanol is recovered, what is ...

Calculate the increase in the boiling point deltat for a

Calculate the increase in the boiling point, ΔT, for a 1.87 m solution of sucrose (dissolves as a molecule) in water.

Identify the chemical components and the proportions

Identify the chemical components and the proportions necessary to achieve a buffer with the desired pH. Include relevant calculations in your answer.

The freezing point of a 1 molar nonelectrolyte such as

The freezing point of a 1 molar nonelectrolyte such as ethylene glycol is. What is the freezing point in degrees C of a 1.79 molar potassium chloride solution? Calculate the osmolarity of a solution made from a 1.876 % ( ...

At 518degc the rate of decomposition of a sample of gaseous

At 518°C, the rate of decomposition of a sample of gaseous acetaldehyde, initially at a pressure of 363 Torr, was 1.07 Torr s-1 when 5.0% had reacted and 0.76 Torr s-1 when 20.0% had reacted. If the reaction rate is prop ...

Directionsrefer back to equation 2 and consider carefully

Directions: "Refer back to equation (2), and consider carefully whether you could simply have used the pH value of the solution at the very beginning of the titration, before any base had been added, to determine either ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

WalMart Identification of theory and critical discussion

Drawing on the prescribed text and/or relevant academic literature, produce a paper which discusses the nature of group

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro