A change in the primary sequence of a protein will never change how a protein is allosteric regulated. a.) True b.) False Which of the following is NOT a mechanism of enzyme regulation? a.) Feedback inhibition by a product. b.) Addition or removal of phosphate groups to amino acids with alcohol groups. c.) Stimulation by a substrate or product. d.) Proteolytic cleavage. e.) Removal of a methyl group from the side chain of alanine. Which of these is NOT true about the active site of an enzyme? a.) It is composed of distinct amino acids that perform catalytic activities b.) It often has a prosthetic group depending on the enzyme c.) It is formed by the folding of the protein d.) It is the site of binding of a competitive inhibitor e.) It will never be affected by changes in the primary structure of the p Which is NOT true about the Km of an enzyme: a.) is calculated as half of the maximal velocity of an enzyme b.) is a hypothetical number that cannot be achieved experimentally c.) is negatively affected by a competitive inhibitor d.) is a measure of how tightly an enzyme binds to its substrate e.) all of the above are true Which is true about Vmax: a.) is the maximal velocity at which an enzyme can function b.) is reduced by a non-competitive inhibitor c.) is determined on a Lineweaver-Burke plot by taking the reciprocal of the y-intercept d.) is low for an enzyme with a low Km and high substrate affinity e.) all of the above