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A back titration method was employed to find out the concentration of Ca2+ ion in a cell culture medium at pH 10.0 alpha4 = 0.85. In this experiment, 25.00mL of 0.1500 M of EDTA was added to 15.00mL of the unknown Ca2+ solution as well as the EDTA that is in excess was titrated using 0.05000 M of Mg2+. It took 5.40mL of Mg2+ solution to reach the equivalence point. Compute the concentration of Ca2+ ion in the unknown.

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