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1) What is the overall charge state for the tetrapeptide DREAM at physiological pH. The pK values for N-terminal, C-terminal, side chain of asp, arg, glu are 9.0, 3.5, 3.9, 12.5, and 4.1, respectively. Note: DREAM is the sequence of Asp-Arg-Glu-Ala-Met

2) Calculate fractional saturation of Mb (K = 2.8) at pO2 = 5, 10, 15, 20.

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