1. What is the functional significance of cooperative binding of oxygen to hemoglobin, for delivery in tissues and for uptake in the lungs? How does this compare with myoglobin binding and delivery of oxygen to tissues?
2. Discuss the models developed to explain cooperative binding of ligands to multisubunit assemblies like oxygen binding to hemoglobin.
3. What is the role of 2, 3-bisphosphoglycerate in the binding affinity of oxygen to hemoglobin?
4. Explain the Bohr Effect. How does low pH and high temperature affect oxygen binding to hemoglobin?
5. Why are hemoglobin-based oxygen carriers not as effective in the binding and delivery of oxygen? Summarize the article by Buchler and Ayalash on the use of hemoglobin-based oxygen carriers.
6. In the paper by Xiao et al. how was the enzyme plasmepsin V characterized? Summarize the study and specify the fragment of the protein used in the study, whether native or recombinant protein was used, and the types of substrates and inhibitors used. What mutations were studies and what features (Km, kcat, Vmax...) were examined in the kinetic analysis of the enzyme. What was the purpose of the study? What conclusions were obtained from the study?
7. Conduct a BLAST search on the protein sequence shown below: At the website, click on PROTEIN BLAST, copy and paste the sequence, select non-redundant protein sequences, select Quick BLASTP. Enter a title for your BLAST search.
a) Summarize the information provided in the readout page and identify the protein with the highest match.
b) Is there complete homology in the matches obtained in the sequence alignment? Based on the encoded amino acids and the organisms shown, are the homologs paralogs or orthologs?
c) In non-homologous areas of the sequence, are there more conserved amino acid substitutions or non-conserved amino acid substitutions?
d) Change some of the parameters on the search page and describe the changes in data retrieval
e) Select at least 2 YouTube tutorials (click YouTube "How to read this page" and "BLAST report description" to view and summarize how the information from the videos can help refine your search.
f) How is the information on the BLAST results page similar or different from what is discussed in chapter 6 of the textbook?
Attachment:- Assignment File.rar