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1.) What is the approximate molecular weight of a protein with 682 amino acid residues in a single polypeptide chain?

2.) The octapeptide AVGWRVKS was digested with the enzyme trypsin. Would ion exchange or molecular exclusion be most appropriate for the separation? Explain. Suppose that the peptide were digested with chymotrypsin. What would be the optimal separation technique? Explain.

 

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