1. The GFP associated with entry P42212 is derived from which source?

• Renilla reniformis
• Aequorea victoria
• Anguilla japonica
• Chiridius poppei

2. Using 7 significant figures, what is the molecular weight of this GFP?

• 26,668.32
• 26,869.36
• 26,886.32
• 26,886

3. Why is the monoisotopic mass different than the average mass?

• The monoisotopic mass is calculated using the averaged mass from all isotopes.
• The monoisotopic mass excludes the N-terminal amino acid.
• The monoisotopic mass is calculated using only the most abundant isotope.
• The monoisotopic mass does not include prosthetic groups.

4. What is the pI of the intact GFP?

• 3.79
• 3.67
• 5.55
• 5.24

5. Removal of the ______ residue(s) would yield a GFP fragment with a lower pI.

• first two
• first five
• first three
• first

6. Which of the 20 standard amino acids is most abundant in GFP?

• K
• G
• D
• T

7. Which of the 20 standard amino acids is least abundant in GFP?

• W
• O
• C
• U

8. GFP contains ____ sulfur atoms, and _____ of these atoms are found in cysteine.

• 6; 0
• 8; 2
• 8; 6
• 6; 10

9. According to the primary page for entry P42212 (under the PTM/processing heading), which of the following GFP residues are modified to yield the fluorescent component (or chromophore) that emits green light when excited?

• Residues 65 and 72
• Only residue 67
• Residues 65 and 67
• Residues 65, 66, and 67