1 in a common protein analysis a dye binds to the protein, Ask an Expert

Academics

1. In a common protein analysis, a dye binds to the protein and the color of the dye changes from brown to blue. The intensity of the blue color is proportional to the amount of protein present.

Protein (µg): 0.00(blank) 9.36 18.72 28.08 37.44
Absorbance: 0.466 0.676 0.883 1.086 1.280

a) After subtracting the blank absorbance (0.466) from the remaining absorbances, use the method of least squares to determine the equation of the best straight line through these 5 points (n = 5). Use the standard deviation of the slope and intercept to express the equation in the form
y (±sy) = [m (±sm)]x + [b(±sb)] with a reasonable number of significant figures.

View complete question

Chemistry, Academics

Category:- Chemistry
Reference No.:- M91099127

Have any Question?Write your Review or question?

Related Questions in Chemistry

What are transition metals and what specifically happens to

What are Transition Metals and what specifically happens to transition metals that results in solutions that give off distinct colors?

A 440 g sample of an unknown metal at 990nbspoc was placed

A 44.0 g sample of an unknown metal at 99.0 o C was placed in a constant-pressure calorimeter of negligible heat capacity containing 80.0 mL water at 24.0 o C. The final temperature of the system was found to be 28.4 ...

An 80-proof brandy is 400 vv ethyl alcohol the proof is

An 80-proof brandy is 40.0% (v/v) ethyl alcohol. The "proof" is twice the percent concentration of alcohol in the beverage. How many milliliters of alcohol are present in 750mL of brandy?

Label each of the following phase changes as either

Label each of the following phase changes as either exothermic or endothermic. i. H 2 O( s ) + heat H 2 O( l ) (1 point) ii. H 2 O( g ) H 2 O( l ) + heat (1 point)

What is the molarity of a solution made by dissolving 200 g

What is the molarity of a solution made by dissolving 20.0 g of CrCl2 in 0.150 L of solution?

How many formula units of barium fluoride baf2 are in 163g

How many formula units of barium fluoride (BaF2) are in 1.63g of BaF2 How many formula units of aluminum chloride (AlCl3) are in 0.582g of AlCl3 How many grams of caffeine (C8H10N4O2) contain 2.53×10^24 molecules of caff ...

Assignment application planned change in a department or

Assignment: Application: Planned Change in a Department or Unit Health care organizations are continuously immersed in change from the emergence of new policies, to promote quality care and improve patient safety to keep ...

How many moles of magnesium ion are present in 525g

How many moles of magnesium ion are present in 5.25g Mg 3 (PO 4 ) 2 ?

A 43 kg person drinks 430 g of milk which has a caloric

A 43 kg person drinks 430 g of milk, which has a "caloric" value ofapproximately 3.0 kJ/g. If only 15% ofthe energy in milk is converted to mechanical work, how high (inmeters) can the person climb based on this energy i ...

When benzene c6h6 reacts with bromine br2 bromobenzene

When benzene (C6H6 ) reacts with bromine (Br2 ), bromobenzene (C6H5Br ) is obtained: C6H6+Br2→C6H5Br+HBr What is the theoretical yield of bromobenzene in this reaction when 28.0 g of benzene reacts with 60.7 g of bromine ...

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Chemistry Expert