(Answered) 1 complete the following purification table which step

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1. Complete the following purification table. Which step gives the best purification?

2. Define and/or explain the significance of All of the following.

a. Explain why a fluorescent amino acid, whether naturally occurring (tryptophane) or artificial (Aladan), might be of advantage in Biochemistry.

Step	Total VoIn me	Total Protein (mg)	Total Activity (units)	Specific Activity (units/mg)	Yield (%)	Purification (n-fold)
Crude Cell extract	2,800	70,000	2,700
Ammonium Sulfate	3,000	25,400	2,300
Heat Treatment	3,000	16,500	1,930
DEAE Chromatography	80	390	1,630
CM Cellulose Chromatography	50	47	1,350
Bio-Gel A Chromatography	7	35	1,120

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