Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Chemical Engineering Expert

problem: Enzyme kinetics

For an enzyme that follows Michealis-Menten kinetics, the initial velocity at different substrate concentration has been determined.

2414_enzyme kinematics_1.jpg

a) Determine KM and VMAX.

b) The experiments are replicated with addition of a reversible inhibitor (the new results are shown in below table). Determine the effect of the inhibitor on KM and VMAX and the type of inhibition.

1099_enzyme kinematics_2.jpg

c) find out the enzyme concentration in the reaction mix, when the turnover number of the enzyme, kcat is 250 s-1.

problem: Batch fermentation

For aerobic cultivation of yeast the cell mass concentration, Xt, and the concentration of the limiting substrate component (glucose), St, has been determined.

1415_Batch fermentation.jpg

a) find out the average cell mass yield factor, YX/S in this fermentation.

b) find out the maximum specific growth rate, μMAX, in the logarithmic growth phase.

c) find out the volumetric growth rate, dX/dt = rX at time=125 min.

d) find out the volumetric substrate utilization rate, rS, and the specific substrate utilization rate, qS, of the yeast at time = 170 min, assuming that the average yield factor can be used to determine rS.

e) find out the concentration of left over substrate at time = 130, again assuming that the average yield factor can be used.
 
problem: Continuous (chemostat) fermentation

A genetically modified yeast is cultivated at pH = 3.3 and temperature = 37oC). Values of substrate flow, F, cell mass concentration, X1, concentration of glucose, S1 (the limiting substrate component), and product concentration, P1, was measures throughout fermentation. The product is a protein – not a primary metabolite – and the substrate utilization for product formation, rS = YX/P,stoich . qp . X, is so low that it can be neglected. All samples were taken after steady state had been established. The feed stream, S0, contains 50.0 g glu/l and no product (P0 = 0), and the working volume, V, is 500 ml. ke = kd = 0.

596_continous fermentation.jpg

a) describe ke and kd.

b) Examine if the growth if following Monod kinetics and if this is the case determine KS and μMAX.

c) Examine if the substrate utilization kinetics can be expressed by growth constants YX/S, true and mS and if this is the case find out these constants.

d) At D = 0.46 h-1 and same conditions as given above find out YX/S, rX, rS, rP, and YP/S.

Chemical Engineering, Engineering

  • Category:- Chemical Engineering
  • Reference No.:- M9303

Have any Question? 


Related Questions in Chemical Engineering

Question 1 a give three examples for each of natural free

Question 1. (a) Give three examples for each of natural (free) convection and forced convection which occur in industrial processes. (b) The Grashof number and the Reynolds number appear in most correlations of experimen ...

Application capacitor with space charge between plates the

Application: Capacitor with space charge between plates. The parallel plate capacitor in Figure 6.41 is given. In addition to the data in Problem 6.1, there is also a charge density everywhere inside the material such th ...

A shell-and-tube heat exchanger with one shell pass and two

A shell-and-tube heat exchanger with one shell pass and two tube passes employs condensing steam at 1 atm on the shell side to heat 10 kg/s of engine oil from 50 to 60°C in the tubes. The steam enters as saturated vapor ...

A draw an arbitrary closed contour on figure 711 and show

(a) Draw an arbitrary closed contour on Figure 7.11 and show that it yields zero net flow of current. (b) Suppose a capacitor is inserted into the circuit instead of any of the resistors and the current is switched on. D ...

Two stirred tanks of volume v and 2v are available for

Two stirred tanks of volume "V" and "2V" are available for carrying out a first order irreversible reaction at constant density and temperature. If the flow rate of feed stream is "Q" which of the following arrangement w ...

The total capital investiment for a chemical plantis 1

The total capital investiment for a chemical plantis $1 million, and the working capital is $100,000. if the plant can produce an average of 8000kg of fixed product per dayduring 365-day per year, what selling price in d ...

Steam enters a converging nozzle at 800 kpa and 55315 k

Steam enters a converging nozzle at 800 kPa and 553.15 K (280°C) with negligible velocity. If expansion is isentropic, what is the minimum pressure that can be reached in such a nozzle and what is the cross-sectional are ...

A horizontal 25-mm diameter cylinder is maintained at a

A horizontal, 25-mm diameter cylinder is maintained at a uniform surface temperature of 35°C. A fluid with a velocity of 0.05 m/s and temperature of 20°C is in cross flow over the cylinder. Determine whether heat transfe ...

A wing generates a lift l when moving through sea-level air

A wing generates a lift L when moving through sea-level air with a velocity U. How fast must the wing move through the air at an altitude of 35,000 ft if it is to generate the same lift? (Assume the lift coefficient is c ...

A well is producing from a saturated oilreservoir with an

A well is producing from a saturated oilreservoir with an average reservoir pressureof 3000 psig. Stabilized flow test dataindicates that the well is capable of producing400 STB/day at a bottom-holeflowing pressure of 25 ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro

Describe what you learned about the impact of economic

Describe what you learned about the impact of economic, social, and demographic trends affecting the US labor environmen