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Two proteins are mixed together in a buffer at pH 6.0. If protein X has a pI of 4.5 and protein Y has a pI of 7.7, determine the net charge of each protein (positive, negative or neutral) at pH 6.0. Next, determine which protein (X, Y, X and Y, or neither) will be retained by an anion-exchange column. In this question, you will need to look up what ion-exchange chromatography is to assist you. pls show work :)

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