Project : Characterizing Genes (An Experimental Design Project)

Addresses course outcome:

• Select and apply knowledge of cellular and molecular processes and current laboratory techniques to effectively participate in the design of scientific studies

The goal of this project is to develop a mouse model system for studying how mutations in the murine (mouse) IGF-1 gene might impact body size and lifespan in the mouse.

Question

As part of this project, your goals is to:

Clone the mouse IGF-1 gene. You will need to discover how to isolate the IGF-1 gene from a mouse and place the gene into a vector for further analysis and use. Remember, you need to isolate the gene, not the mRNA/cDNA