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Imagine you're translating artificially created RNAs using a cell-free system like that developed in Nirenberg's lab. The first batch of RNA is made by mixing together A and G nucleotides in a 3:1 ratio (3 A : 1 G) and randomly linking them together. The second batch is made the same way, but instead is made from a pool of A and U nucleotides (3 A : 1 U). Naturally the amino acid content of the polypeptides that are synthesized will be different, but how ELSE will the polypeptides produced from these two different RNA mixtures differ?

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