(Answered) How would you make up 10 ml of the stock pcr buffer from

Management Studies

How would you make up 10 mL of the stock PCR buffer from the following stock solutions?

1 M Tris HCl pH 8.3
2 M KCl
183 mM MgCl₂
0.12 %(w/v) Gelatin
100 mM for each of the dNTPs; dATP, dGTP, dCTP and dTTP

The stock PCR buffer contains:

12.2 mM Tris HCl (pH 8.3),
61 mM KCl,
1.83 mM MgCl₂
0.0012% (w/v) gelatin,
244 μM dNTPs

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