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Botulinus toxin causes paralysis. It binds to presynaptic sites at the motor end plate (neuromuscular junction) and prevents the exocytotic release of transmitter from vesicles. Predict the consequences (increase, decrease, no change & explain why) of blocking the release of acetylcholine at the neuromuscular junction on the following: 1. Presynaptic resting potential 2. Presynaptic action potential amplitude 3. Presynaptic Ca2+ influx 4. Presynaptic transmitter release 5. Postsynaptic resting potential 6. Postsynaptic end plate potential 7. Postsynaptic action potential amplitude 8. Postsynaptic action potential frequency 9. Muscle contractile strength

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