Process Synthesis:

A process for isolating an antibody against insulin has, a unit operation, the reaction of the antibody with the antigen in a continuous stirred tnk reactor. The reaction product is a precipitate that is continuously removed from the reactor with 10% of the solution, which is mouse serum. Since insulin is an expensive reagent, only stoichiometric amounts can be added to the mouse serum, which contains 8 mg/liter of anti-insulin. If this particular monoclonal antibody precipitates with its antigen in a 1:1 ratio, how many milligrams of insulin must be added to the reactor per hour to process 100ml of mouse serum per hour. (Assume that equilibrium is achieved in this reactor). Molecular weight of mouse insulin is 5,808 Da. Molecular weight of monocolonal antibody is 6,000 Da. (Student thinks we can assume that the insulin-mAb complex is 13,809 Da).